Pathogen flora in milk - 4 criteria♦ - 2025

Proficiency testing

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Introduction

The proficiency testings and criteria marked with an are covered by the « accréditation n° 1-2473, comparaisons interlaboratoires, portée disponible sur www.cofrac.fr »/« n° 1-2473 accreditation, interlaboratory comparisons, scope available on www.cofrac.fr », and detailed flexible scope available there

 

- We propose tests for the enumeration of Listeria monocytogenes in milk and cheese which must not be mistaken for tests for detection of Listeria.

- The tests for the detection of Salmonella do not concern Salmonella Typhi and Paratyphi.

- The enumeration of sulfito-reducing Clostridium spp., Clostridium perfringens and Bacillus cereus concerns non-spore-forming bacteria. The analysis must be carried out without heat treatment.

In accordance with ISO 15213-1:2023, “ASR bacteria” is used if no confirmation step of characteristic colonies is carried out. If characteristic colonies are confirmed, the “Clostridium spp. sulfito-reducing agents” term is then applied.

- We draw your attention to the fact that we don’t contaminate our samples with collection strains but with strains isolated from dairy food matrices. Therefore, it is possible that some of them present unexpected phenotypic characteristics on some culture media.


Methods / Analytes

enumeration of Listeria spp., Listeria monocytogenes, enterobacteria (at low rate) and detection of Salmonella

For Listeria and Salmonella: free choice methods. For Enterobacteria: a MPN method is recommended. For the enumeration on dishes, do not use ready-to-use rehydratable medium.


Number of samples

→ 5 samples containing between 0 and 10 000 Listeria/ml

→ 5 samples for the detection of Salmonella in 25 ml of milk

→ 5 samples containing between around 0 and 100 enterobacteria/ml

→ 1 vial of water to control the temperature of the samples at reception


Packaging

- five 30 ml vials for the enumeration of Listeria spp. including Listeria monocytogenes and/or enumeration of Listeria monocytogenes
- five 60 ml vials for the detection of Salmonella
- five 30 ml vials for the enumeration of enterobacteria
All come in screw-capped vials with airtight seals


Preservation

storage at 3 (±2) °C, with a bacteriostatic mixture preventing any bacterial growth in the milk, but rendered inactive upon dilution

CAUTION no bacterial growth occurs in the undiluted sample with ready-to-use rehydratable medium (because of the bacteriostatic mixture). Therefore do not use this type of medium for enterobacteria.


Dispatch

in IATA certified secondary packaging (biohazard), in insulated box with ice packs by express carrier (see regulation for shipping times)


Storage in the laboratory

at 3 (± 2) °C 


Validity of the samples

analyses must be carried out within 3 days after the date of dispatch


Calendar of dispatch :

 

Sample dispatch 2025/12/09
Return of results to ACTALIA Cecalait 2025/12/23

Subscription at least 10 days before each dispatch

Rates

Members Non-members
Registration fees per shipment 74.09 € 111.1 €
Participation fee per tested method 74.09 € 111.1 €


Special conditions :

  • For groups from 10 to 24 participating laboratories: 10 % discount,
  • For groups from 25 participating laboratories: 15 % discount

Payment conditions :

within 1 month of receiving invoice

Formulas

  • Enmeration of Listeria spp.
  • Enumeration of Listeria monocytogenes
  • Detection of Salmonella
  • Enumeration of Enterobacteriaceae




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Contact us

ACTALIA Cecalait

Rue de Versailles
BP 70129
39801 POLIGNY CEDEX
FRANCE

Tel : +33 (0)3 84 73 63 20
Fax : +33 (0)3 84 73 63 29

cecalait[at]actalia.eu

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