The proficiency testings and criteria marked with an ♦ are covered by the « accréditation n° 1-2473, comparaisons interlaboratoires, portée disponible sur www.cofrac.fr »/« n° 1-2473 accreditation, interlaboratory comparisons, scope available on www.cofrac.fr », and detailed flexible scope available there
In accordance with ISO 15213-1:2023, “ASR bacteria” is used if no confirmation step of characteristic colonies is carried out. If characteristic colonies are confirmed, the “Clostridium spp. sulfito-reducing agents” term is then applied.
- We draw your attention to the fact that we don’t contaminate our samples with collection strains but with strains isolated from dairy food matrices. Therefore, it is possible that some of them present unexpected phenotypic characteristics on some culture media.
The fat content of the cheese to be analyzed is less than 20%
- detection of Listeria♦ and Salmonella♦,
- enumeration of coagulase positive Staphylococci♦, Escherichia coli♦, microorganisms at 30 °C♦, enterobacteria♦, ASR / sulfito-reducing Clostridium spp.♦, Clostridium perfringens♦ and Bacillus cereus♦
free choice for methods
→ 5 samples containing between about:
- absence or presence of Listeria in 25g of cheese and identification of the species, included Listeria monocytogenes
- absence or presence of Salmonella in 25g of cheese
- 0 and 10 000 coagulase positive staphylococci/g
- 0 and 100 000 Escherichia coli/g
- 10 000 and 500 000 microorganisms at 30 °C/g
- 0 and 100 000 enterobacteria/g
- 0 and 1 000 ASR / sulfito-reducing Clostridium spp. (without thermisation)/g
- 0 and 1 000 Clostridium perfringens (without thermisation)/g
- 0 and 30 000 Bacillus cereus (without thermisation)/g
→ 1 vial of water to control the temperature of the samples at reception
about 100 g in 120 ml safety vials with locked lids
storage at 3 (±2) °C, with a bacteriostatic mixture preventing any bacterial growth in the milk, but rendered inactive upon dilution
in IATA certified secondary packaging (biohazard), in insulated box with ice packs by express carrier (see regulation for shipping times)
at 3 (± 2) °C
analyses must be carried out within 3 days after the date of dispatch
| Sample dispatch | 2025/03/11 | 2025/09/16 |
| Return of results to ACTALIA Cecalait | 2025/03/25 | 2025/09/30 |
Subscription at least 10 days before each dispatch
| Members | Non-members | |
|---|---|---|
| Registration fees per shipment | 73.37 € | 109.99 € |
| Participation fee per tested method | 73.37 € | 109.99 € |
within 1 month of receiving invoice
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ACTALIA Cecalait
Rue de Versailles
BP 70129
39801 POLIGNY CEDEX
FRANCE
Tel : +33 (0)3 84 73 63 20
Fax : +33 (0)3 84 73 63 29
Accreditation no 1-5577, tests, no 1-2473 interlaboratory comparisons, scope available on www.cofrac.fr/en
